Journal: International Journal of Clinical and Experimental Medicine

Article Title: p53 mediated apoptosis in osteosarcoma MG-63 cells by inhibition of FANCD2 gene expression

doi:

Figure Lengend Snippet: FANCD2 protein expression in MG-63 cells after RNAi. Lane 1: Control; Lane 2: siRNA-Control; Lane 3: siRNA-FANCD2 24 h; Lane 4: siRNA-FANCD2 48 h.

Article Snippet: Construction and transfection of the FANCD2 siRNA in MG-63 cells siRNA-FANCD2 and a control siRNA plasmid were designed and synthesized by Santa Cruz Biotechnology, Inc. (Texas, USA).

Techniques: Expressing, Control

Journal: International Journal of Clinical and Experimental Medicine

Article Title: p53 mediated apoptosis in osteosarcoma MG-63 cells by inhibition of FANCD2 gene expression

doi:

Figure Lengend Snippet: Absorbance of MG-63 cells after siRNA-FANCD2 interfere by CCK-8 assay ( x ̅ ±s, n = 6)

Article Snippet: Construction and transfection of the FANCD2 siRNA in MG-63 cells siRNA-FANCD2 and a control siRNA plasmid were designed and synthesized by Santa Cruz Biotechnology, Inc. (Texas, USA).

Techniques: CCK-8 Assay, Control

Journal: International Journal of Clinical and Experimental Medicine

Article Title: p53 mediated apoptosis in osteosarcoma MG-63 cells by inhibition of FANCD2 gene expression

doi:

Figure Lengend Snippet: Cell cycle distribution of MG-63 cells after siRNA-FANCD2 interfere ( x ̅ ±s, n = 4)

Article Snippet: Construction and transfection of the FANCD2 siRNA in MG-63 cells siRNA-FANCD2 and a control siRNA plasmid were designed and synthesized by Santa Cruz Biotechnology, Inc. (Texas, USA).

Techniques: Control

Journal: International Journal of Clinical and Experimental Medicine

Article Title: p53 mediated apoptosis in osteosarcoma MG-63 cells by inhibition of FANCD2 gene expression

doi:

Figure Lengend Snippet: Apoptotic percentages of MG-63 cells after FANCD2 siRNA interfere by Flow Cytometry ( x ̅ ±s, n = 4)

Article Snippet: Construction and transfection of the FANCD2 siRNA in MG-63 cells siRNA-FANCD2 and a control siRNA plasmid were designed and synthesized by Santa Cruz Biotechnology, Inc. (Texas, USA).

Techniques: Flow Cytometry, Control

Journal: International Journal of Clinical and Experimental Medicine

Article Title: p53 mediated apoptosis in osteosarcoma MG-63 cells by inhibition of FANCD2 gene expression

doi:

Figure Lengend Snippet: Relative expression of mRNAs in MG-63 cells after FANCD2 siRNA interfere ( x ̅ ±s, n = 3)

Article Snippet: Construction and transfection of the FANCD2 siRNA in MG-63 cells siRNA-FANCD2 and a control siRNA plasmid were designed and synthesized by Santa Cruz Biotechnology, Inc. (Texas, USA).

Techniques: Expressing, Control

Journal: International Journal of Clinical and Experimental Medicine

Article Title: p53 mediated apoptosis in osteosarcoma MG-63 cells by inhibition of FANCD2 gene expression

doi:

Figure Lengend Snippet: Western blotting picture of p53, phos-p53, p21, TP53INP1, cleaved caspase-9 and-3 protein expression after RNAi. Lane 1: Control; Lane 2: siRNA-Control; Lane 3: siRNA-FANCD2 24 h; Lane 4: siRNA-FANCD2 48 h.

Article Snippet: Construction and transfection of the FANCD2 siRNA in MG-63 cells siRNA-FANCD2 and a control siRNA plasmid were designed and synthesized by Santa Cruz Biotechnology, Inc. (Texas, USA).

Techniques: Western Blot, Expressing, Control

Journal: BMC Cancer

Article Title: FANCD2 inhibits ferroptosis by regulating the JAK2/STAT3 pathway in osteosarcoma

doi: 10.1186/s12885-023-10626-7

Figure Lengend Snippet: Primers for qRT-PCR in this study

Article Snippet: The human osteosarcoma cell lines (MG-63 and U2OS) and osteoblast cells (hFOB1.19) were obtained from Chinese Academy of Sciences Cell Bank (Shanghai, China), and cultured in DMEM medium (Invitrogen, Carlsbad, CA, USA) containing 10% FBS, 1% streptomycin/penicillin at 37 °C in an atmosphere of 5% CO 2 . siRNA targeting FANCD2 (si-FANCD2) and si-NC were purchased from RiboBio (Shanghai, China).

Techniques: Sequencing

Journal: BMC Cancer

Article Title: FANCD2 inhibits ferroptosis by regulating the JAK2/STAT3 pathway in osteosarcoma

doi: 10.1186/s12885-023-10626-7

Figure Lengend Snippet: FANCD2 is up-regulated in osteosarcoma cells. Relative protein expression of FANCD2 in osteosarcoma cells was determined by western blot. ** P < 0.01 versus hFOB1.19

Article Snippet: The human osteosarcoma cell lines (MG-63 and U2OS) and osteoblast cells (hFOB1.19) were obtained from Chinese Academy of Sciences Cell Bank (Shanghai, China), and cultured in DMEM medium (Invitrogen, Carlsbad, CA, USA) containing 10% FBS, 1% streptomycin/penicillin at 37 °C in an atmosphere of 5% CO 2 . siRNA targeting FANCD2 (si-FANCD2) and si-NC were purchased from RiboBio (Shanghai, China).

Techniques: Expressing, Western Blot

Journal: BMC Cancer

Article Title: FANCD2 inhibits ferroptosis by regulating the JAK2/STAT3 pathway in osteosarcoma

doi: 10.1186/s12885-023-10626-7

Figure Lengend Snippet: FANCD2 silencing inhibits cell proliferation, migration, and invasion of osteosarcoma cells. (A) FANCD2 mRNA expression was detected by RT-qPCR. (B) FANCD2 protein expression was determined by western blot; the membranes were cut prior to hybridisation with antibodies. (C) Cell viability of MG-63 and U2OS cells was measured by MTT assay. (D) The proliferation of MG-63 and U2OS cells was detected by the EdU detection kit. E-F. The migration and invasion of MG-63 and U2OS cells were determined by wound healing and transwell assay. ** P < 0.01 versus si-NC

Article Snippet: The human osteosarcoma cell lines (MG-63 and U2OS) and osteoblast cells (hFOB1.19) were obtained from Chinese Academy of Sciences Cell Bank (Shanghai, China), and cultured in DMEM medium (Invitrogen, Carlsbad, CA, USA) containing 10% FBS, 1% streptomycin/penicillin at 37 °C in an atmosphere of 5% CO 2 . siRNA targeting FANCD2 (si-FANCD2) and si-NC were purchased from RiboBio (Shanghai, China).

Techniques: Migration, Expressing, Quantitative RT-PCR, Western Blot, Hybridization, MTT Assay, Transwell Assay

Journal: BMC Cancer

Article Title: FANCD2 inhibits ferroptosis by regulating the JAK2/STAT3 pathway in osteosarcoma

doi: 10.1186/s12885-023-10626-7

Figure Lengend Snippet: FANCD2 silencing predisposes ferroptosis. (A) The levels of iron, ferrous iron, and LIP were determined by iron detection kits and calcein-acetoxymethyl ester method, respectively. (B) The expression of ferroptosis-related genes (FTH1, GPX4, and COX2) was determined by RT-qPCR. (C) MDA, SOD, and CAT content were determined by the appropriate kits. ** P < 0.01 versus si-NC. ## P < 0.01 versus si-FANCD2

Article Snippet: The human osteosarcoma cell lines (MG-63 and U2OS) and osteoblast cells (hFOB1.19) were obtained from Chinese Academy of Sciences Cell Bank (Shanghai, China), and cultured in DMEM medium (Invitrogen, Carlsbad, CA, USA) containing 10% FBS, 1% streptomycin/penicillin at 37 °C in an atmosphere of 5% CO 2 . siRNA targeting FANCD2 (si-FANCD2) and si-NC were purchased from RiboBio (Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR

Journal: BMC Cancer

Article Title: FANCD2 inhibits ferroptosis by regulating the JAK2/STAT3 pathway in osteosarcoma

doi: 10.1186/s12885-023-10626-7

Figure Lengend Snippet: FANCD2 interacts with the JAK2/STAT3 pathway. The expression of JAK, p-JAK, STAT3, and p-STAT3 was determined by western blot. ** P < 0.01 versus si-NC

Article Snippet: The human osteosarcoma cell lines (MG-63 and U2OS) and osteoblast cells (hFOB1.19) were obtained from Chinese Academy of Sciences Cell Bank (Shanghai, China), and cultured in DMEM medium (Invitrogen, Carlsbad, CA, USA) containing 10% FBS, 1% streptomycin/penicillin at 37 °C in an atmosphere of 5% CO 2 . siRNA targeting FANCD2 (si-FANCD2) and si-NC were purchased from RiboBio (Shanghai, China).

Techniques: Expressing, Western Blot

Journal: BMC Cancer

Article Title: FANCD2 inhibits ferroptosis by regulating the JAK2/STAT3 pathway in osteosarcoma

doi: 10.1186/s12885-023-10626-7

Figure Lengend Snippet: Colivelin attenuates the inhibition effects of FANCD2 silencing on malignant phenotype of osteosarcoma cells. (A) Cell viability of U2OS cells was determined by MTT assay. (B) The proliferation of U2OS cells was detected by the EdU detection kit. C-D. Wound healing and transwell assay were used to analyze U2OS cell migration and invasion. * P < 0.05, ** P < 0.01 versus si-NC. # P < 0.05, ## P < 0.01 versus si-FANCD2

Article Snippet: The human osteosarcoma cell lines (MG-63 and U2OS) and osteoblast cells (hFOB1.19) were obtained from Chinese Academy of Sciences Cell Bank (Shanghai, China), and cultured in DMEM medium (Invitrogen, Carlsbad, CA, USA) containing 10% FBS, 1% streptomycin/penicillin at 37 °C in an atmosphere of 5% CO 2 . siRNA targeting FANCD2 (si-FANCD2) and si-NC were purchased from RiboBio (Shanghai, China).

Techniques: Inhibition, MTT Assay, Transwell Assay, Migration

Journal: BMC Cancer

Article Title: FANCD2 inhibits ferroptosis by regulating the JAK2/STAT3 pathway in osteosarcoma

doi: 10.1186/s12885-023-10626-7

Figure Lengend Snippet: Colivelin reverses the effects of FANCD2 silencing on ferroptosis. (A) The levels of iron, Fe 2+ , and LIP were determined by iron detection kits and calcein-acetoxymethyl ester method. (B) RT-qPCR was used to detect the expression of ferroptosis-related genes. (C) The levels of MDA, SOD, and CAT were detected by the appropriate kits. ** P < 0.01 versus si-NC. # P < 0.05, ## P < 0.01 versus si-FANCD2. & P < 0.05, && P < 0.01 versus Erastin

Article Snippet: The human osteosarcoma cell lines (MG-63 and U2OS) and osteoblast cells (hFOB1.19) were obtained from Chinese Academy of Sciences Cell Bank (Shanghai, China), and cultured in DMEM medium (Invitrogen, Carlsbad, CA, USA) containing 10% FBS, 1% streptomycin/penicillin at 37 °C in an atmosphere of 5% CO 2 . siRNA targeting FANCD2 (si-FANCD2) and si-NC were purchased from RiboBio (Shanghai, China).

Techniques: Quantitative RT-PCR, Expressing

Journal: BMC Cancer

Article Title: FANCD2 inhibits ferroptosis by regulating the JAK2/STAT3 pathway in osteosarcoma

doi: 10.1186/s12885-023-10626-7

Figure Lengend Snippet: FANCD2 silencing inhibits tumor growth. (A) Tumor tissue images and tumor volume were measured in different groups. (B) Tumor weight was assessed in different groups. ** P < 0.01 versus Lv-sh-NC. (C) IHC staining of FANCD2 expression in tumor tissues of Lv-shNC and Lv-shFANCD2 groups. (D) The expression of ferroptosis-related genes in tumor tissues was detected by RT-qPCR

Article Snippet: The human osteosarcoma cell lines (MG-63 and U2OS) and osteoblast cells (hFOB1.19) were obtained from Chinese Academy of Sciences Cell Bank (Shanghai, China), and cultured in DMEM medium (Invitrogen, Carlsbad, CA, USA) containing 10% FBS, 1% streptomycin/penicillin at 37 °C in an atmosphere of 5% CO 2 . siRNA targeting FANCD2 (si-FANCD2) and si-NC were purchased from RiboBio (Shanghai, China).

Techniques: Immunohistochemistry, Expressing, Quantitative RT-PCR